Table 1- Testing Protocol
|Description of Purpose|
|1||Off||Off||Control:||Measure survival of Ps. fluorescens in water that has passed through the system KDF off by inoculating after collection.|
|Measure survival in water that has passed through KDF by inoculating after collection.|
|3||On||Off||Control:||Measure survival of Ps. fluorescens that have been injected into the system with KDF off.|
|Measure survival of Ps. fluorescens that have come in direct contact with KDF.|
All water used for testing was deionized and injected with AOAC synthetic hard water to a final concentration of 100 ppm and a pH 7.0 +/-.2
Following collection, the samples were surface plated on Plate Count Agar within 2 to 3 minutes of collection and again after incubation at room temperature for 3 hours.
The same inoculums of Pseudomonas fluorescens was used for injection in to Samples 3 and 4 or for addition to Samples 1 and 2 (added after collection of the water).